About the National BioResource Project (Yeast)
Overview and Objectives
Yeast is one of the premier industrial microorganisms, because of its essential role in brewing, winemaking, baking, and fuel alcohol production. In addition, yeast has proven to be an excellent model organism for the study of a variety of biological problems involving the fields of genetics, molecular biology, cell biology and other disciplines within the biomedical and life sciences.

The complete genomes of the budding yeast (Saccharomyces cerevisiae) and the fission yeast (Schizosaccharomyces pombe) have been determined and those of other yeasts are being sequenced at breath-taking speed. A major focus of current yeast research concerns genome-wide functional analysis-the development of tools and methods to decipher the functions of all the genes of an organism under a variety of environmental conditions. -- On-going groundbreaking work in the functional genomics of the budding and fission yeasts will assure their importance as model eukaryotes in the foreseeable future.

Resources such as yeast strains and DNA clones are absolutely necessary for a wide variety of yeast research. In the past, such resources were stored by individual researchers and were distributed by request. Establishment of a central yeast genetic resource center has been a goal of yeast researchers for many years in order to overcome the inherent inefficiency and inequities of such a system. Thus, the National BioResource Yeast Project was launched in 2002 by the Japanese Ministry of Education, Culture, Sports, Science and Technology.

The purpose of the project is the systematic collection, storage, and dissemination of important yeast genetic resources including strains, plasmids, DNA libraries, and antibodies.
The physical infrastructure of the Yeast Genetic Resource Center (YGRC) is located at Osaka City University and Osaka University to provide the aforementioned services. A Steering Committee has been established to develop operational policies and to assure open communication between the Center and the yeast research community at large. An Advisory Board evaluates the activities of the YGRC on an annual basis and provides guidance and advice concerning various aspects of the project.


Steering Committee
AKADA RinjiYamaguchi University, Graduate School of Medicine
ARAKI HiroyukiNational Institute of Genetics, Division of Microbial Genetics
OYA YoshikazuThe University of Tokyo, Graduate School of Frontier Science( Chairman )
KANEKO YoshinobuOsaka University, Graduate School of Engineering( Subject Director )
KAWAMUKAI MakotoShimane University, Faculty of Life and Environmental Science
KITAMURA KenjiHiroshima University, Natural Science Center for Basic Research and Development( Subject Director )
KITAMOTO HirokoNational Institute for Agro-Environmental Sciences
SAKUNO TakeshiThe University of Tokyo, Institute of Molecular and Cellular Biosciences
SHIMODA ChikashiOsaka City University, Graduate School of Science
TAKEGAWA KaoruKyushu University, Graduate School of Bioresource and Bioenvironmental Sciences
TOHDA HidekiAsahi Glass Co., Ltd.
TSUCHIYA EikoHiroshima University, Graduate School of Advanced Sciences of Matter
NAKAZAWA NorihikoOkinawa Institute of Science and Technology Graduate University
NAKAMURA TaroOsaka City University, Graduate School of Science( Representative )
NISHIZAWA MasafumiKeio University, School of Medicine
HARASHIMA SatoshiOsaka University, Graduate School of Engineering( Subject Director )
HIRAOKA YasushiOsaka University, Graduate School of Frontier Biosciences
FUKUDA HisashiNational Research Institute of Brewing, Fundamental Research Division
MAEKAWA HiromiOsaka University, Graduate School of Engineering
MORIYA HisaoOkayama University, Research Core for Interdisciplinary Sciences
YAMAGISHI HiromiAsahi Breweries, LTD., Corporate Planning Division
YAMASHITA AkiraNational Institute for Basic Biology, Laboratory of Cell Responses

Advisory Board
OSHIMA YasujiOsaka University
TOH-E AkioThe University of Tokyo
MIYAGAWA TokichiHiroshima University
YANAGIDA MitsuhiroOkinawa Institute of Science and Technology Graduate University
YAMAMOTO MasayukiNational Institute for Basic Biology

Yeast Genetic Resource Center (YGRC)
The two most useful yeast species, Schizosaccharomyces pombe (fission yeast) and Saccharomyces cerevisiae (budding yeast), are managed by research groups at Osaka City University and Osaka University, respectively. These two groups collaborate closely in order to fulfill the goals of the project.
Fission yeast
Yeast Genetic Resource Center (YGRC), Graduate School of Science, Osaka City University

Nakamura, Taro (Head, Professor)
Shimoda, Chikashi (Professor)
Nakahara, Fumiko (Technical Assistant)

Sugimoto 3-3-138, Sumiyoshi-ku, Osaka
Phone: +81 66605 2576
Fax: +81 66605 3158

Current status of resource storage:
S. pombe strains: ~13,000
Genomic and cDNA libraries
Plasmids: ~96,000
Budding yeast
Yeast Genetic Resources Lab, Graduate School of Engineering, Osaka University

Kaneko, Yoshinobu (Head, Endowed Chair Professor)
Maekawa, Hiromi (Endowed Chair Assoc. Professor)
Harashima, Satoshi (Professor)
Zhou, Ying (Researcher)

Yamadaoka 2-1, Suita, Osaka
Phone&Fax: +81 66879 7422

Current status of resource storage:
S. cerevisiae strains: ~24,400
Genomic library and Plasmids: ~4,100

About our activities
An overview of the YGRC is illustrated as follows:

How to deposit resources:
If you wish to deposit strains or plasmids, please contact the YGRC by E-mail.
Fission yeast ()
Budding yeast ()
Initially, a Material Transfer Agreement (MTA) will be signed and exchanged, in which you will indicate your preferred terms for future distribution of provided materials. You will then be asked to send the materials and to provide as much background information as possible (i.e., restriction maps, details about mutant isolation or construction, etc.) in order to make the materials a useful resource for future users. In the case of large contributions, the YGRC will send technical personnel to your laboratory to facilitate the transfer of materials.

Storage/Quality inspection:
Strains are stored in glycerol at -80oC. When new strains are deposited, phenotypes of most are confirmed. Strains harboring new combinations of mutant alleles are also constructed at the YGRC.

Construction and use of the databases:
Information about most strains and plasmids stored at the YGRC is available online and is searchable based on genotype or other relevant parameters.

Dissemination of yeast genetic resources:
If you wish to obtain yeast strains, plasmids or libraries, please contact us by E-mail. After receiving the materials you will be asked to complete, sign, and return to us an appropriate MTA. Depending on the materials, you may need to provide us with an agreement signed in advance by the Intellectual Property Right (IPR) holder.