What's New and Topics (What's New?)
2017/7/28 Budding yeast strains used in researches for autophagy and TOR kinases were deposited by Dr. Y. Kamada (National Institute for Basic Biology, Japan). These strains (BY28944-28996) are now available (Kamada et al., (2000), Kawamata et al., (2008), Kamada et al., (2010), Nakashima et al., (2008), Kamada, (2017), Kamada et al., (2005)).
2017/7/28 Ogataea polymorpha strains used in genome editing by CRISPR/Cas were deposited by Dr. Y. Kaneko (Osaka University, Japan). These strains (BY28908-28943) are now available (Numamoto et al., (2017)).
2017/5/15 Genome-wide segmental aneuploids of budding yeast were deposited by Dr. S. Harashima (Sojo University, Japan). These strains (BY28643-28692) are now available (Natesuntorn et al., (2015)).
2017/5/8 Multiple deletion strains of budding yeast protein phosphatase genes PTC1-PTC7 were deposited by Dr. S. Harashima (Sojo University, Japan). These strains (BY28520-28617) are now available (Sharmin et al., (2014)).
2017/4/28 Budding yeast mutants related to inositol biosynthesis were deposited by Dr. J. Nikawa (Kyushu Institute of Technology, Japan). These strains (BY19544-19567) are now available (Sugiyama et al., (2001)).
2017/4/27 Fission yeast plasmids related to GFP-binding protein were deposited by Dr. Jin (Xiamen University). These plasmids (FYP3955-3984) are now available (Chen et al., 2017).
2016/12/06 Fission yeast resources related to exocytic pathway and sporulation (Strains: FY25555-25608, 25629-25667, 25758-25825, 28902-28954 and Plasmids: FYP3496-3504 etc) are now available (Imada and Nakamura, 2016).
2016/12/04 Fission yeast strains (FY29132-29607) and plasmids (FYP3792-3815) related to meiotic chromosome segregation, used in Yamamoto, A. and Hiraoka, Y. (2003), Hirose et al. (2011), Yoshida et al. (2013), Matsuhara and Yamamoto (2016) and Katsumata et al. (2016), are deposited by Dr. A. Yamamoto (Shizuoka Univ., Japan).
2016/12/04 Fission yeast strains (FY31339-31344) used in Nakazawa et al. (2015) deposited by Dr. M. Yanagida (OIST, Japan).
2016/10/17 Hybrid sake yeast strains constructed through a new crossbreeding approach were deposited by Dr. N. Fukuda (National Institute of Advanced Industrial Science and Technology (AIST), Japan). These strains (BY28505-28519) are available on request.
2016/10/11 Plasmids (BYP8941-8951) deposited by Dr. Y. Kaneko (Osaka University, Japan) are now available on request. These plasmids were used in a genetic study of PHO pathway in yeast Hansenula polymorpha.
2016/09/29 Wild fission yeast strains whose origin are different from Leupold L968 are deposited by Dr. J. Bahler (University College London, UK) . These strains (FY28955-29092) are available on request.
2016/09/28 Several cell cycle mutants of fission yeast are deposited by Dr. K. L. Gould (Vanderbilt University School of Medicine, USA). These strains (FY29093-29131) are available on request.
2016/06/14 The barcode yeast strains (BY27873-28253) deposited by Dr. S. Rutherford (Fred Hutchinson Cancer Research Center, USA) are now available on request. These strains were used in a study of yeast responses to osmotic stress.
2016/05/06 TOR-related strains (FY25826-25872) used in Ikai et al. (2011) deposited by Dr. M. Yanagida (OIST, Japan) is now available.
2016/03/14 90 gene knock out mutants are added to the fission yeast deletion mutant set deposited by Dr. K. Gould (Vanderbilt University, USA). They are not contained in the deletion mutant library set ver 3 (Bioneer corporation, Korea).
2016/03/09 A new fission yeast deletion set (90 strains) deposited by Dr. K. Gould (Vanderbilt University School of Medicine, USA) is now available for distribution(FY25668-25757).
2016/01/20 Thiamine-repressible expression vectors based on the backbone of the pREP-3X, pREP-41X and pREP-81X plasmids (Yeast. 2000;16(9):861-72.) deposited by Dr. C. Ribas (Univ. of Salamanca, Spain) are now available (18 plasmids, FYP3462-3479).
2016/01/07 Budding yeast strains (BY28357-BY28502) deposited by Dr. A. Nakano (RIKEN, Japan) have been available.
2015/12/11 Budding yeast strains (BY28260-BY28295) and plasmids (BYP8875-BYP8880) deposited by Dr. S. Tanaka (National Institute of Genetics, Japan) have been available.
2015/10/27 Fission yeast plasmids (FYP2932-2935) deposited by Dr. H. Nojima (Osaka Univ.) are now available.
2015/10/27 pTN575 (FYP2931), the vector of the cDNA library pTN-FC10, is now available.
2015/10/23 Two cDNA libraries, pTN-FC9 and pTN-FC10, are now available. These libraries contain cDNA, about 90% of which are full-length cDNA.
2015/10/09 Order of fission yeast strain sets are now available.
2015/10/09 We started to provide eMTA creation system.
2015/09/17 Kluyveromyces marxianus mutants (BY28297-BY28356; Yeast 30:485, 2013; Yeast 27:29, 2010) and plasmids (BYP8881-BYP8884; Yeast 31:29, 2014) were deposited by Dr. H. Hoshida (Yamaguchi University, Japan). They are now available on request.
2015/09/10 11cassettes for single-step drug-resistance and prototrophic marker switching in fission yeast (Yeast 2015) by Dr. A. Lorenz (Univ. of Aberdeen, UK) are now available (FYP2884-2894).
2015/07/15 35 'modules', including promoters, fluorescent tags and selection markers, to construct various plasmids in a one-step manner (Open Biol. 2015 Jun;5(6).) deposited by Dr. M. Sato (Waseda Univ.) are now available (FYP2895-2929).
2015/07/15 8 integrating vectors with mutated adh1 promotors deposited by Dr. Y. Watanabe (Univ. of Tokyo) are now available (FYP2874-2881).
2015/06/08 Fission yeast strains (FY17852-FY17875) deposited by Dr.T. Nakamura are now available.
2015/06/08 Fission yeast strains (FY25312-FY25336) deposited by Dr. M. Yanagida (OIST) are now available.
2015/5/22 Fission yeast strains (FY23587-FY23592, FY23594, FY23596, FY23601-FY23604, FY23606-FY23610, FY23616-FY23619, FY23625, FY23626, FY23676-FY23680) deposited by Dr. T. Sugiyama (NIH) are now available.
2015/05/12 Non-standard S. pombe strains are now available. (FY25283-FY25301, FY25303-FY25311)
2015/04/28 Fission yeast strains (FY24008-25242, FY25257-FY25275) deposited by Dr. Y. Watanabe (University of Tokyo) are now available.
2015/04/28 Fission yeast strains (FY25243-25248) deposited by Dr. H.Murakami (Chuo University) are now available.
2015/04/28 Fission yeast strains deposited by Dr. J. Mata (Univ. Cambridge, UK) are now available for distribution(FY25251-FY25256).
2014/09/16 A new fission yeast deletion set deposited by Dr.K. Gould (Vanderbilt University School of Medicine, USA) is now available for distribution (FY23693-23986).
2014/09/16 Fission yeast strains deposited by Dr. R. Weismann (Open University of Israel, Israel) are now available for distribution(FY23988-24006).
2014/08/28 pFA6a plasmids carrying drug-resistant markers, (Nat, Ble or Hyg) deposited by Dr. A.W. Carr (University of Sussex, UK) are now available (FYP2869-2871).
2014/06/11 Budding yeast vectors (pRS42x; Gene 110:119, 1992) deposited by Dr. H. Moriya (Okayama University, Japan) are now available. The vectors are BYP7838 to BYP7841.
2014/05/09 Fission yeast mutants (structural genes for pheromone) are now available (FY22789-22972).
2014/04/14 Budding yeast resources related to ribosome biogenesis (deposited by Dr. K. Mizuta, Hiroshima University, Japan) are now available. Strains are BY26955 to BY26963 and plasmids are BYP7851 to BYP7871.
2013/10/15 Fission yeast ts and cs mutant sets are now available (FY22064-22708). These mutants are deposited by Dr. T. Tani (Kumamoto University).
2013/10/15 Fission yeast mug gene disruptant set is deposited by Dr. S. Moreno (Salamanca University).
2013/10/15 Fission yeast pheromone-related gene disruptants are now available.
2013/10/11 Budding yeast mutants deposited by Dr. H. Hoshida (Yamaguchi University, Japan) are now available. (BY25957-BY26000, BY26944-BY26954; Yeast 30:243, 2013)
2013/07/24 Budding yeast expression vectors (BYP7579-BYP7588) deposited by Dr. J. Ishii (Kobe Univ., Japan) and gTOW vectors (BYP7589, BYP7590) deposited by Dr. H. Moriya (Okayama Univ., Japan) are now available.
2013/06/12 Fission yeast strains (FY21635-21664) deposited by Dr. H. Tohda (Asahi Glass Co. Ltd, Japan) are now available.
2013/05/29 Fission yeast strains (FY21828-21832) deposited by Dr. K. Nakano (Tsukuba University) are now available.
2013/03/18 Auxin-inducible-degron plasmids (BYP7425 - BYP7435; Mol. Cell, 2013 http://dx.doi.org/10.1016/j.molcel.2013.02.012) deposited by Dr. M. Kanemaki (National Institute of Genetics, Japan) are now available in the budding yeast resources.
2013/02/25 Budding yeast mutants related to ribosome biogenesis (BY25930-BY25946; deposited by Dr. K. Mizuta, Hiroshima University, Japan) and drug hypersensitive pdr1 pdr3 double mutant (BY25929; deposited by Dr. M. Ueki, RIKEN, Japan) are now available.
2012/12/10 A series of PGK1-promoter-expression vectors for the budding yeast (BYP7357 - BYP7373) was deposited by Dr. J. Ishii (Kobe University, Japan) and now available.
2012/08/09 Budding yeast plasmids for stress granules markers (BYP7348 - BYP7356) were deposited by Dr. S. Izawa (Kyoto Institute of Technology, Japan) and now available.
2012/05/25 Budding yeast plasmids related to ribosome biogenesis (BYP7304 - BYP7324) were deposited by Dr. K. Mizuta (Hiroshima University, Japan) and now available.
2012/03/16 Budding yeast auxin-inducible degron strains (BY25917 to BY25927) deposited by Dr. M. Kanemaki (National Institute of Genetics, Japan) and GFP-fusion plasmids for visualization of P-body in the budding yeast (BYP7215 and BYP7216) deposited by Dr. S. Izawa (Kyoto Institute of Technology, Japan) are now available.
2012/01/31 Fission yeast strains (FY21444-21460) deposited by Dr. S. Sazer (Baylor College of Medicine) are now available.
2012/01/31 Fission yeast strains (FY21462-21477) deposited by Dr. S. Hauf (Friedrich Miescher Laboratory of the Max Planck Society) are now available.
2011/12/12 Heat-inducible degron system for S. pombe (Plasmids, FYP2779-2786)deposited by Prof. Stephen Kearsey (Oxford University) is now available.
2011/11/25 Auxin-inducible degron system for S. pombe (Strains; FY21102,21103,21104, and Plasmid, FYP2827) deposited by Prof. Masukata (Osaka University) is now available.
2011/05/10 Septin-related budding yeast strains (BY25624 to BY25887) deposited by Dr. M. Iwase (University of Tokyo, Japan) are now available.
2011/02/25 Budding yeast septin-related plasmids (BYP6854 to BYP7144) deposited by Dr. M. Iwase (University of Tokyo, Japan) are now available.
2011/02/23 Fission yeast strains (24 strains; FY19908-FY19931) deposited by Dr. C. Shimoda (Osaka City University, Japan) are now available.
2011/02/23 Fission yeast strains (23 strains; FY19781-FY19806) deposited by Drs. H. Nojima and D. Okuzaki (Osaka University, Japan) are now available.
2011/02/23 Fission yeast strains (284 strains; FY19055-FY19373) deposited by Dr. J. Bahler (University College London, London, UK) are now available.
2011/02/08 Ribosome biosynthesis-related mutants (BY25900 to BY25915) was deposited by Prof. K. Mizuta (Hiroshima University, Japan) and are available now for distribution.
2011/01/14 Now sequences of full length cDNAs are available.
2010/12/28 Budding yeast strains expressing tagged proteasome subunit (BY25889 to BY25899) were deposited by Dr. Y. Saeki (Tokyo Metropolitan Institute of Medical Science, Japan) and are now available.
2010/12/17 From April 2009 onwards, the Genome Viewer was displaying incorrect information about S. pombe genomic DNA clones. We apologize for this error and would like to offer a retraction.Specifically, DNA clones mapped to after 3.7Mbp of chromosome 1 were wrongly displayed by a maximum of 6150bp. This was caused by the usage of an older version of genome sequence to analyze the homologous region of DNA clones.As a result, if you are using clones mapped to after 3.7Mbp of chromosome 1,there is a possibility that the desired gene is not included in the clone.The resource center is going to contact and resend the correct clones for all previous orders but if you are in a hurry, please contact us.We will strive to avoid such mistakes in the future and we hope to receive your continuous support. Yukiko Yamazaki of NBRP Information Center
2010/12/01 Double promoter (TEF1 and PGK1)-vector plasmids (BYP7150 and BYP7151; Yeast 27:955, 2010) was deposited by Prof. Jens Nielsen (Chalmers University of Technology, Sweden) and are now available.
2010/11/11 Fission yeast strains (337 strains) deposited by Dr. J. Kohli (Bern University, Switzerland) are now available.
2010/11/11 S. pombe plasmids for gene-tagging/-fusion deposited by Dr. S. Forsburg (Univ. Southern California) are now available.
2010/09/02 Auxin-inducible-degron related plasmids (BYP6851 - BYP6853; Dr.M. Kanemaki, Osaka Univ., Japan), Fluorescent protein-expressed strains (BY25610 – BY25612; M. Carmi, Weizmann Inst. of Science , Israel) and rad mutants (BY25614 – BY25623; YGRC) are available now for distribution.
2009/12/01 Dr.M. Kanemaki (Osaka Univ., Japan) has deposited the auxin-inducible degron system and its related strains (BYP6739 – BYP6744, BY255576 – BY25603), which were published in Nature Methods online (doi: 10.1038/nmeth.1401).
2009/10/01 Akada's budding yeast genomic library clones (BYP6618-BYP6738) and budding yeasts (BY25550-BY25557) from R. Akada (Yamaguchi Univ) are available for disitribution.
2009/08/19 Budding yeast plasmids (BYP6604 - BYP6617) from Dr.A. Sakai (MITILS) are available for distribution.
2009/05/20 Fission yeast strains (FY17884-FY17930) from Dr.T. Tani (Kumamoto Univ) and from Teresa Wang (Stanford Univ) were added to our collection.
2009/04/17 Akada's budding yeast genomic library clones (BYP6475-BYP6538) and OUT budding yeasts (BY19901-BY19992) are available for disitribution.
2009/03/17 Ninety-eitht fission yeast full-length cDNA clones are added to our collection (FYC1484-FYC1581).
2009/03/06 Dr.H. Takagi (NAIST, Japan) has deposited the budding yeast mutants and plasmids (Genes Cells 13:105, 2008). BYP6472-BYP6474: HA-tagged HSF1, MSN2, and MSN4. BY25535-BY25541: rsp5 mutant and related strains.
2009/01/23 Dr.Akada’s library clones carrying budding yeast genes (BYP6413-BYP6469; 57 plasmids) and double disruptants of protein phosphatase gene (SIW14 or PPZ1) and protein kinase gene (BY10001-BY10202) are available for distribution.
2008/12/25 Fission yeast strains (FY17835-FY17851) from Dr.H. Nojima (Osaka) and from Dr.N. Hamasaki-Katagiri (Maryland) were added to our collection.
2008/11/25 A set of GFP-fusion strains (1,060 strains; FY14458-FY15517) of fission yeast are now available for distribution. These strains were constructed by Yasushi Hiraoka's lab (Osaka Univ./NICT).
2008/11/18 Fission yeast strains (FY17729-17834) are deposited by Dr.Yasushi Hiraoka (Osaka Univ. / KARC), Hiroshi Murakami (Nagoya City Univ.) and Dr.Makoto Kawamukai (Shimane Univ.).
2008/10/07 Akada’s library clones carrying budding yeast genes (BYP6337 - BYP6412; 76 plasmids) are available for distribution.
2008/09/03 Fission yeast strains (FY17641-17728, 84 strains) are deposited by Dr.Takashi Toda(Cancer Research UK). These strains have been reported in recent issues of Nature (PMID17476213) and Nature Cell Biol (PMID17486116).
2008/08/26 Fission yeast GFP-tagging strains constructed by Yasushi Hiraoka's Lab are added to our resource collection.
2008/08/12 New budding yeast resources, i.e., [BYP6238-BYP6254, BYP6256-BYP6318; pAK plasmids] from A. Toh-e (Rinshoken), [BYP6327-BYP6334; J. Biol. Chem. 280:35822, 2005] from Dr.Y. Kikuchi (Univ. of Tokyo) & Dr.Y. Takahashi (NIH, USA), and [BYP6335 and BYP6336, plasmids for split-GFP method; Fungal Genet. Biol. 45:597-604 (2008)] from D. J. Timson (Queen's Univ.), are available for distribution.
2008/06/17 S. pombe full-length cDNA clones are added (Release-3; 42 clones). These full-length cDNAs are derived from mRNA mixtures prepared from vegetative and meiotic cells of h90 strain L968. This cDNA library is constructed by Vector-Capping method(Kato et al., 2005 DNA Research 12: 53-62). You may search the clones by ‘systematic ID’ or ‘gene symbol’ as a query. If you find any desired cDNAs you are welcome to order them online. The ordered materials are to be sent in a week or so with nucleotide sequence data.
2008/06/04 S. pombe genomic DNA clones are now available for distribution. Please click "Physical Maps" on this page, then you may find genomic clones mapped on chromosomes. Random Sau3A fragments from a wild-type S. pombe strain L972 were cloned into the pAL-KS vector. Order by E-mail (nbrpombe@sci.osaka-cu.ac.jp) is welcome.
2008/05/30 New fission yeast strains [FY17627-17637] from M. Yanagida and R. Sugiura (kinki Univ.) are available for distribution. S. pombe plasmids (8 clones) from Taro Nakamura (Osaka City Univ.) are added to the collection.
2008/05/29 New fission yeast strains [FY17237-17355]from M. Yanagida (50 strains; Kyoto Univ.), T. Tani (8 strains; Kumamoto Univ.) and T. Nakamura (50 strains; Osaka City Univ.) are available for distribution.
2008/03/06 S. pombe full-length cDNA clones are added (Release-2, 482 clones). These full-length cDNAs are derived from mRNA mixtures prepared from vegetative and meiotic cells of h90 strain L968. This cDNA library is constructed by Vector-Capping method(Kato et al., 2005 DNA Research 12: 53-62). You may search the clones by ‘systematic ID’ or ‘gene symbol’ as a query. If you find any desired cDNAs you are welcome to order them online. The ordered materials are to be sent in a week or so with nucleotide sequence data.
2008/02/25 New fission yeast strains [FY17233-FY17236] from M. Ueno (Hiroshima Univ.) are available for distribution.
2008/02/08 New budding yeast resources, [BYP6237, expression plasmid] from R. Akada (Yamaguchi Univ.) and [BYP5346-BYP5584, TOp plasmids (first batch)] from A. Toh-e (Rinshoken), are available for distribution.
2008/01/09 New budding yeast resources, [BYP6221-BYP6236, expression plasmids] from R. Akada (Yamaguchi Univ.) and [BY25525-BY25530, aryl hydrocarbon-reporter yeasts] from M. Kawanishi (Osaka Prefecture University), are available for distribution.
2007/12/26 New fission yeast strains [FY17223-17232] and plasmids [FYP2224-2229] from Kentaro Nakano (Tsukuba Univ.) and from Takayoshi Kuno (Koe Univ.), respectively, are available for distribution.
2007/12/07 New budding yeast resources, [BY25470-BY25473, ste mutants] from R. Akada (Yamaguchi Univ.) and [BY25520-BY25524, SUMO related mutants] from Y. Takahashi & Y. Kikuchi (NIH, USA & Univ. of Tokyo), are available for distribution.
2007/11/12 S. pombe full-length cDNA clones are now available (Release-1, 958 clones). These full-length cDNAs are derived from mRNA mixtures prepared from vegetative and meiotic cells of h90 strain L968. This cDNA library is constructed by Vector-Capping method(Kato et al., 2005 DNA Research 12: 53-62). You may search the clones by ‘systematic ID’ or ‘gene symbol’ as a query. If you find any desired cDNAs you are welcome to order them online. The ordered materials are to be sent in a week or so with nucleotide sequence data. Release-2 will come in a few weeks.
2007/11/06 New budding yeast resources (BY25450-BY25469, Sake yeast derivatives) from R. Akada (Yamaguchi Univ.) are available for distribution.
2007/10/24 New fission yeast plasmids are deposited by Y. Hama (Asahi Glass Co.), M. Yanagida (Kyoto Univ.) and A. Ishihama (Hosei Univ.). They are available for distribution [FYP2027-2217].
2007/10/19 New budding yeast resources [BY21796-BY21799 (K. lactis), BY25348-BY25449, BYP6160-BYP6204, BYP6215-BYP6216, BYP6217-BYP6220 (plasmids for K. lactis)] are available for distribution.
2007/10/11 S. pombe strains carrying six new amino acid-auxotrophic markers deposited by T. Kuno are available for distribution (Curr Genet 52: 97-105, 2007). Suitable for gene disruption.
2007/08/03 NBRP-Yeast is linked to GeneDB (S. pombe) and SGD (S. cerevisiae) through the genetic symbols marked with underline at the result page of strain retrieval.
2007/06/13 Large-scale-deletion budding yeast strains (BYP21770 - BYP21787; Appl. Microbiol. Biotechnol. 75:589, 2007) are available for distribution, except for BY21770, BY21771 and BY21779.
2007/05/08 Akada DNA library (BYP6127 - BYP6145; Mol. Gen. Genet. 254:267, 1997) is now available for distribution in the budding yeast plasmid resources.
2007/04/16 We renewed this site.
2007/04/11 Protease-deficient S. pombe mutants deposited by Dr. Y. Hama (ASPEX Division,Asahi Glass Co. Ltd.) are now available for distribution (FY16976-16979).